METHOD FOR PROMOTING DISULFIDE BOND FORMATION IN RECOMBINANT PROTEINS

Reduced cysteine-containing proteins consisting of recombinant IFN-.beta., IL-2 muteins thereof may be oxidized selectively so that the recombinant proteins have essentially the same disulfide bridging and biological activity as their native counterparts. The oxidized product is substantially free o...

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Hauptverfasser:	KOTHS, KIRSTON E, HALENBECK, ROBERT F
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY ELECTRIC COMMUNICATION TECHNIQUE ELECTRICITY METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PICTORIAL COMMUNICATION, e.g. TELEVISION THEIR RELEVANT APPARATUS TRANSPORTING
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Beschreibung
Zusammenfassung:	Reduced cysteine-containing proteins consisting of recombinant IFN-.beta., IL-2 muteins thereof may be oxidized selectively so that the recombinant proteins have essentially the same disulfide bridging and biological activity as their native counterparts. The oxidized product is substantially free of unwanted side products and contains a minimal amount of intermolecular oligomers. The oxidation takes place in an aqueous medium containing a solubilizing agent at a pH of about 5.5 to 9, preferably at a pH of about 7. The reaction is initiated by addition of at least an effective amount of an oxidation promoter containing a Cu+2 cation such as CuCl2 or ophenanthroline/Cu+2 complex in the presence of air.