REAGENT AND PROCESS FOR THE DETERMINATION OF THE ACTIVITY OF THE ENZYME Y-GLUTAMYL TRANSFERASE

Process and reagent for the determination of .gamma.-glutamyl transferase The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a coloured compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity. The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents: .gamma.-GT-arylamide 1 - 100 mmol/l. .gamma.-glutamyl acceptor (glycylglycine) 10 - 500 mmol/l. coupling component 1 - 500 mmol/l. oxidation agent 0.1 - 500 mmol/l. buffer 10 - 1000 mmol/l.