PROCESS AND ANALYTICAL AGENT FOR THE DETERMINATION OF THE ENZYME GLUTAMATE-OXALACETATE-TRANSAMINASE

Process and analytical agent for the determination of the activity of glutamate-oxalacetate-transaminase The present invention provides a process for the determination of the activity of glutamateoxalacetate-transaminase (GOT) in aqueous solutions in which a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalysed by GOT, to give pyruvate, sulphur dioxide and glutamate, b) the resultant pyruvate is reacted with phosphate and oxygen, catalysed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen pexoxide, c) the hydrogen peroxide and a chromogen is reacted, catalysed by a peroxidase, to give a dyestuff and water and d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphoxlyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-¢4-dimethylaminophenyl!-2-¢3,5-dimethoxy-4-hydroxyphenyl!-imidazole (B-DDH-imidazole) or a related substance. The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.-ketoglutarate, alanine-sulphinic