DIPHENYLHYDANTOIN DERIVATIVES

ABSTRACT OF THE DISCLOSURE Reagents for use in binding assays, particularly immuno-assays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including .beta.-galactosyl-umbelliferone-diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N1, N3 and .sigma.-phenyl derivatives of diphenylhydantoin for preparing same. N1 and N3-.omega.- aminoalkyl and .sigma.-(.omega.-aminoalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to a .beta.-galactosyl-umbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N1 and N3-.omega.-carboxyalkyl and .sigma.-(.omega.-carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic poly-amino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.