WAVELENGTH-CORRECTED SPECTROFLUOROMETER

A spectrofluorometer providing wavelength correction by means of an arbitrary function generator driven by a wavelength scanning element of the spectrofluorometer. The arbitrary function generator may be driven by the emission scanning monochromator to derive a wavelength-dependent voltage signal which is combined with the measure photomultiplier output to thereby provide a base-line compensated resultant output signal. Alternatively, the arbitrary function generator may be driven by the excitation scanning monochromator to derive a wavelength-dependent voltage signal which is combined with the output of a reference photomultiplier tube receiving part of the output of the excitation monochromator to derive an excitation-corrected reference signal which is in turn combined with the measure photomultiplier tube output signal to thereby provide an energy-corrected resultant output signal. An analog divider may be employed to divide this last-named resultant output signal by said excitation wavelength-dependent voltage signal to thereby derive a quantum-corrected fluorescence emission output signal. The arbitrary function generator has successive incremental wavelength range segments provided respectively with independently adjustable potentiometers to suitably shape the wavelength-dependent voltage signal curve.