"método para analisar metabólitos polares e método para arrefecer bruscamente uma amostra biológica que compreende material celular"

The present invention is concerned with the analysis of polar metabolites and provides methods for analyzing polar metabolites comprising extracting a biological sample with a extraction buffer comprising a phase separator and a volatile neutral ammonium salt under conditions which allow for immedia...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: GERD BALCKE, MARTIN DOSTLER, TILMANN B. WALK, RALF LOOSER, SUSAN CARVALHO
Format: Patent
Sprache:por
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Beschreibung
Zusammenfassung:The present invention is concerned with the analysis of polar metabolites and provides methods for analyzing polar metabolites comprising extracting a biological sample with a extraction buffer comprising a phase separator and a volatile neutral ammonium salt under conditions which allow for immediate disruption of cells comprised by the biological sample, separating the polar metabolites comprised by the extract by chromatography, and analyzing the separated polar metabolites. Moreover, a method for quenching a biological sample comprising cellular material is contemplated.