processo aperfeiçoado para peguilacao de proteínas

processo aperfeiçoado para peguilacao de proteínas

The present invention relates to a process for improving pegylation reaction yield of r-metHuG-CSF comprising conjugating r-metHuG-CSF to a PEG aldehyde at a free amine moiety at the N terminal end on the G-CSF in presence of a reducing agent in a pegylation buffer solution comprising a polyol havin...

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Hauptverfasser: MURALIDHARAN CHANDRAKESAN, RAKESH SHEKHAWAT, ARUNA KHARE, PANKAJ PRABHAKAR MALPURE, DIVYA LAL SAKSENA, DILIP KUMAR RANA, NIKHIL UMESH MOHE, RADHARKRISHNAN VENKATSUBRAMANIAN TARUR, SAGAR SATYANARAYAN ZAWAR, DIGAMBER SHRIPATI PAWAR, DINESHI KUMAR PALIWAL, NILESH DAGDU PATIL
Format: Patent
Sprache:por
Schlagworte:
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Beschreibung
Zusammenfassung:The present invention relates to a process for improving pegylation reaction yield of r-metHuG-CSF comprising conjugating r-metHuG-CSF to a PEG aldehyde at a free amine moiety at the N terminal end on the G-CSF in presence of a reducing agent in a pegylation buffer solution comprising a polyol having the formula CnH2n+2On where n is from 3 to 6, or a carbohydrate, or a derivative thereof wherein the concentration of said polyol or carbohydrate or derivative thereof is in the range of 0.1% to 10% w/w.