MÉTODOS PARA PRODUZIR UM EXTRATO DE PROTEÍNA A PARTIR DE CÉLULAS FIXADAS OU NÃO-FIXADAS E PARA DETECTAR UMA PROTEÍNA ALVO, SISTEMA PARA PRODUZIR UM EXTRATO DE PROTEÍNA, KIT PARA PRODUZIR UM EXTRATO DE PROTEÍNA DE CÉLULAS FIXADAS OU NÃO-FIXADAS, E, MÉTODO PARA EXTRAIR UMA PROTEÍNA VIRAL ALVO DE UMA AMOSTRA CELULAR

Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.