processo para produzir uma vacina da proteìna e2 de pestivìrus recombinante em cultura de células de inseto, e, vacina da subunidade e2

The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in gro...

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Bibliographische Detailangaben
Hauptverfasser:	DIRK FRANCISCUS MARINUS VAN DE WIEL, ABRAHAM JOHANNES DE SMIT, ERIK KEES HAMANN, ROBERTUS JACOBUS MARIA MOORMANN, DIETMAR KRETZDORN
Format:	Patent
Sprache:	por
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a sufficient volume to contain at least 2 liters and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1 x 10 to 5 x 10 cells/ml with an m.o.i of