método de isolamento de lipídios a partir de uma biomassa contendo lipídios

a presente invenção se refere a um método de lipídios contendo ácidos graxos poli-insaturados isolados de uma biomassa contendo lipídios. The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.