oligonucleotídeos modificados compostos de funções tióis e o uso destes para a detecção de ácidos nucleicos

oligonucleotídeos modificados compostos de funções tióis e o uso destes para a detecção de ácidos nucleicos

The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, prefe...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: JEAN-FRANÇOIS CANTALOUBE, JEAN-JACQUES VASSEUR, JULIE MAYEN, MYRIAM LEREAU, CHANTAL FOURNIER-WIRTH, ALBERT MEYER, CAROLE FARRE, CAROLE CHAIX, FRANÇOIS MORVAN
Format: Patent
Sprache:por
Schlagworte:
CHEMISTRY
DERIVATIVES THEREOF
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PHYSICS
SUGARS
TESTING
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Beschreibung
Zusammenfassung:The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, preferably maleimide or acrylamide functions. The invention also relates to a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridization between a modified oligonucleotide and a target nucleic acid amplified from the biological sample. The invention relates more particularly to a method for detecting, genotyping or sequencing a pathogenic organism, preferably a virus.