processo para preparar misturas de reaÇço estabilizadas que sço total ou parcialmente secas, compreendendo pelo menos uma enzima, misturas de reaÇço e kits contendo as referidas misturas

The invention relates to a method consisting of bringing into contact, in one container, (a) an aqueous solution of a reaction mixture comprising at least one enzyme, and (b) an aqueous solution of a stabilizing mixture comprising (i) at least one protective agent against drying, (ii) at least one i...

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Bibliographische Detailangaben
Hauptverfasser: GEMMA LIMONES LOPEZ, MARIA DOLORES MARIN ALBERDI, PEDRO MANUEL FRANCO DE SARABIA ROSADO, ANTONIO MADEJON SEIZ
Format: Patent
Sprache:por
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Beschreibung
Zusammenfassung:The invention relates to a method consisting of bringing into contact, in one container, (a) an aqueous solution of a reaction mixture comprising at least one enzyme, and (b) an aqueous solution of a stabilizing mixture comprising (i) at least one protective agent against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups, and (iii) at least one inert polymer capable of generating a mesh structure preventing the mobility of the dried reagents. The invention also consists of removing all or part of the water contained in the resulting aqueous solution. Said process is suitable for carrying out enzymatic reactions, for example, amplifying, sequencing and characterizing nucleic acids, performing hybridization tests and for the restriction analysis.