Transcriptionally silenced plant genes

The invention relates to gene silencing as observed after integration of transgenes into plant genomes. Comparison of transcriptional gene expression between an Arabidopsis line carrying a silent transgene present in multiple copies and its mutant derivative mom1 impaired in silencing of the transge...

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Bibliographische Detailangaben
Hauptverfasser:	JERZY PASZKOWSKI, ANDREA STEIMER, ORTRUN MITTELSTEN SCHEID
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to gene silencing as observed after integration of transgenes into plant genomes. Comparison of transcriptional gene expression between an Arabidopsis line carrying a silent transgene present in multiple copies and its mutant derivative mom1 impaired in silencing of the transgene revealed two cDNA clones which are expressed in the mutant plants, but not in the parental and not in wild type plants. Both clones are derived from the same family of transcripts referred to as TSI (Transcriptionally Silent Information). Genomic templates encoding TSI are repetitive elements with mainly pericentromeric location and conserved organization among various ecotypes. Transcriptional silencing of the genomic TSI templates is specifically released in the mutant. Transcription of TSI can be used as a marker to identify a defective silencing pathway in a plant.