Methods for detecting and measuring spliced nucleic acids

A simplified method for preparing nucleic acid, preferably spliced mRNA, from a biological sample that is suitable for amplification is disclosed. A method of detecting and measuring the amount of one or more species of spliced mRNA present in the sample, following nucleic acid amplification, is dis...

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Bibliographische Detailangaben
Hauptverfasser:	RICHARD C. HARVEY, PAUL S. EASTMAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A simplified method for preparing nucleic acid, preferably spliced mRNA, from a biological sample that is suitable for amplification is disclosed. A method of detecting and measuring the amount of one or more species of spliced mRNA present in the sample, following nucleic acid amplification, is disclosed.