Plasmids for plant transformation and method for using the same

The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that redu...

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Hauptverfasser: LAMBERTUS HENRICUS SIMONS, SIETSKE HOEKSTRA, ANNE SILENE PONSTEIN, BERNARDUS MARTINUS MARIA DEKKER, HENDRIK TIGELAAR, STEPHAN ANDREAS OHL, MAARTEN HENDRIK STUIVER, OSCAR JOHANNA MARIA GODDIJN, NICOLAS ELZINGA
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creator LAMBERTUS HENRICUS SIMONS
SIETSKE HOEKSTRA
ANNE SILENE PONSTEIN
BERNARDUS MARTINUS MARIA DEKKER
HENDRIK TIGELAAR
STEPHAN ANDREAS OHL
MAARTEN HENDRIK STUIVER
OSCAR JOHANNA MARIA GODDIJN
NICOLAS ELZINGA
description The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
title Plasmids for plant transformation and method for using the same
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