Plasmids for plant transformation and method for using the same

The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that redu...

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Bibliographische Detailangaben
Hauptverfasser:	LAMBERTUS HENRICUS SIMONS, SIETSKE HOEKSTRA, ANNE SILENE PONSTEIN, BERNARDUS MARTINUS MARIA DEKKER, HENDRIK TIGELAAR, STEPHAN ANDREAS OHL, MAARTEN HENDRIK STUIVER, OSCAR JOHANNA MARIA GODDIJN, NICOLAS ELZINGA
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.