Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant

PCT No. PCT/US96/20777 Sec. 371 Date Apr. 15, 1997 Sec. 102(e) Date Apr. 15, 1997 PCT Filed Dec. 13, 1996 PCT Pub. No. WO97/21825 PCT Pub. Date Jun. 19, 1997This invention provides a method for obtaining a recombinant retroviral packaging cell capable of producing retroviral vectors as well as the r...

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Hauptverfasser:	ERNST BOHNLEIN, JONATHAN S. DANDO, JINGYI CHEN, SEAN P. FORESTELL, IVAN PLAVEC, RICHARD J. RIGG
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	PCT No. PCT/US96/20777 Sec. 371 Date Apr. 15, 1997 Sec. 102(e) Date Apr. 15, 1997 PCT Filed Dec. 13, 1996 PCT Pub. No. WO97/21825 PCT Pub. Date Jun. 19, 1997This invention provides a method for obtaining a recombinant retroviral packaging cell capable of producing retroviral vectors as well as the recombinant packaging cell obtained by the method. Also provided is a method of producing recombinant retroviral particles obtained by introducing into the packaging cells obtained according to the methods disclosed herein, a recombinant retroviral vector and propagating the resulting producer cells under conditions favorable for the production and secretion of retroviral vector supernatant. The retroviral supernatant produced by these methods also is claimed herein. This invention further provides a method for screening retroviral vector supernatant for high transduction efficiency and methods for producing retroviral vector supernatant for transducing cells with high efficiency in gene therapy applications.