Peptides for tissue and cell culture media

Peptides for tissue and cell culture media

The invention relates to a method and culture medium for in vitro culturing eucaryotic cells requiring glutamine, which comprises the use of a protein hydrolysate obtained by enzymatic hydrolysis of a protein material. The protein hydrolysate has a free amino acid level of less than 15 % by weight a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: WIM R BLOM, MARCELLINUS JACOBUS J. HAKKAART, GREGORY W LULI, BARTHOLOMEUS JOSEF VAN SCHIE, ANTHONIE KUNST
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to a method and culture medium for in vitro culturing eucaryotic cells requiring glutamine, which comprises the use of a protein hydrolysate obtained by enzymatic hydrolysis of a protein material. The protein hydrolysate has a free amino acid level of less than 15 % by weight and the protein hydrolisate have molecular weight below 44 kD. The protein hydrolysate preferably contains at least 20 % of glutamine residues. Also, preferably at least 90 % by weight of the peptides have a molecular weight below 1000 D and the average peptide chain length preferably is below 15 amino acid residues.