Monoclonal antibodies against soluble TNF-alpha receptors p55 and p75 as well as against TNF-alpha and its analogues

The present invention belongs to the field of pharmaceutical industry and relates to novel monoclonal antibodies and fragments thereof which are bound to the available surface of a human recombinant TNF- alpha , TNF- alpha analogues and TNF- alpha soluble receptors p55 and p75. Further, the inventio...

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Bibliographische Detailangaben
Hauptverfasser:	ANNETTE SCHUURBIERS, VIKTOR MENART, VLADKA CURIN SERBEC, JANJA OCVIRK, VLADIMIRA GADERC POREKAR, BORUT STABUC, PETRA ZUNEC, ANTON STALC
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention belongs to the field of pharmaceutical industry and relates to novel monoclonal antibodies and fragments thereof which are bound to the available surface of a human recombinant TNF- alpha , TNF- alpha analogues and TNF- alpha soluble receptors p55 and p75. Further, the invention relates to stable hybridoma cell lines, which lines are capable of producing such monoclonal antibodies, to a process for the preparation thereof and to pharmaceutical preparations containing them. The invention also relates to a method for the quantitative determination of human TNF- alpha , free or complexed with soluble TNF- alpha receptors p55 and p75, and to a kit used therefor. By hitherto disclosed monoclonal antibodies and reagents developed therefrom, it has not been possible to simultaneously determine free TNF- alpha and its soluble receptors p55 and p75 as well as complexes thereof. Another object of the invention is also the TNF- alpha analogue TNF- alpha Cys95His107His108Cys148, which in a complex with TNF- alpha soluble receptors, is used as an antigen for immunization of mice.