Glucose responsive insulin secreting beta-cell lines and method for producing same

A method of selecting cells with enhanced secretion of a secretory product is disclosed. The method comprises exposing a population of cells to a secretagogue to result in the secretion of a secretory product from the cells and selecting from the population, cells that exhibit increased amounts of i...

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Bibliographische Detailangaben
Hauptverfasser:	DAVID KNAACK, DEBORAH M FIORE, MEGAN E LAURANCE, ORION D HEGRE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of selecting cells with enhanced secretion of a secretory product is disclosed. The method comprises exposing a population of cells to a secretagogue to result in the secretion of a secretory product from the cells and selecting from the population, cells that exhibit increased amounts of intracellular free calcium when exposed to the secretagogue. The method enables the selection of correctly regulated beta cells that secrete appropriate amounts of insulin in response to varying glucose levels.