Fungal resistant plants, process for obtaining fungal resistant plants and recombinant polynucleotides for use therein

Plants PI and PII which exhibit relative overexpression of intracellular chitinase and beta-1,3-glucanase genes resp. are new. The overexpression is a result of genetic manipulation of the plants (or their ancestors) using one or more recombinant polynucleotides. Also claimed are: (1) recombinant po...

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Hauptverfasser: JEROEN SEBASTIAN CHARLES VAN ROEKEL, CHARLES PETER WOLOSHUK, ELISABETH JOSINE SOPHIE MEULENHOFF, HUBERTUS JOSEPHUS MARIA LINTHORST, JOHN FERDINAND BOL, LEO SJOERD MELCHERS, ALEXANDRA ALEIDA VLOEMANS, MARIANNE BEATRIX SELA-BUURLAGE, BERNARDUS JOHANNES CLEMENS CORNELISSEN
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Sprache:eng
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Zusammenfassung:Plants PI and PII which exhibit relative overexpression of intracellular chitinase and beta-1,3-glucanase genes resp. are new. The overexpression is a result of genetic manipulation of the plants (or their ancestors) using one or more recombinant polynucleotides. Also claimed are: (1) recombinant polynucleotides (I) and (II) comprising (a) a promoter functional in plants; (b) a gene encoding the chitinase beta-1,3-glucanase, under control of the promoter; (c) a terminator, operably linked to the gene; and (d) a gene encoding a selectable or screenable trait, operably linked to regulatory sequences for proper expression; (2) a cloning or transformation vector comprising (I) or (II); (3) plasmids pMOG200, pMOG212, pMOG289, pMOG512 and derivs. of these; (4) bacteria harbouring a plasmid as in (3); and (5) a process for obtg. fungal resistant plants by introducing into the genome of the plants, a recombinant polynucleotide as above.