Ubiquitin carrier enzyme E2-F1 purification, production, and use

Ubiquitin carrier enzyme E2-F1 purification, production, and use

Purified isolated E2-F1 enzyme (I) does not bind to anion exchange resin in neutral pH and is capable of stimulating protein degradation of non N-end rule substrates and N-alpha -acetylated substrates. Also claimed are: (1) an antibody which is capable of binding specifically to E2-F1 enzyme; (2) a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: AARON J. CIECHANOVER, NAVA BLUMENFELD, HEDVA GONEN
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Purified isolated E2-F1 enzyme (I) does not bind to anion exchange resin in neutral pH and is capable of stimulating protein degradation of non N-end rule substrates and N-alpha -acetylated substrates. Also claimed are: (1) an antibody which is capable of binding specifically to E2-F1 enzyme; (2) a hybridoma cell line which produces the antibody of (1); (3) a purified and isolated DNA molecule coding for E2-F1 enzyme where the DNA is pref. in the form of an expression vector; (4) a host transformed with the expression vector of (3); and (5) the prodn. of (I) by (a) culturing a recombinant host comprising a gene coding for (I); and (b) isolating (I) from the recombinant host.