Polyvinyl alcohol-based magnetic polymer particles, methods for their preparation and their use

Spherically shaped polyvinyl alcohol (PVAL) polymer particles are prepared encapsulating a magnetic colloid that provides the particles with magnetic properties for use in binding biomolecules. The particles have a size of 1-10 mum that makes them particularly suitable for use in cell separation/sor...

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Bibliographische Detailangaben
1. Verfasser:	DETLEF MULLER-SCHULTE DR
Format:	Patent
Sprache:	eng
Schlagworte:	AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G BASIC ELECTRIC ELEMENTS BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OF MACROMOLECULAR COMPOUNDS COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ELECTRICITY ENZYMOLOGY GENERAL PROCESSES OF COMPOUNDING HUMAN NECESSITIES HYGIENE INDUCTANCES INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS MAGNETS MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY ORGANIC MACROMOLECULAR COMPOUNDS PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES SEPARATION SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS SPIRITS SUGARS TESTING THEIR PREPARATION OR CHEMICAL WORKING-UP THEIR RELEVANT APPARATUS TRANSFORMERS TRANSPORTING USE OF INORGANIC OR NON-MACROMOLECULAR ORGANIC SUBSTANCES ASCOMPOUNDING INGREDIENTS VINEGAR WINE WORKING-UP
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Beschreibung
Zusammenfassung:	Spherically shaped polyvinyl alcohol (PVAL) polymer particles are prepared encapsulating a magnetic colloid that provides the particles with magnetic properties for use in binding biomolecules. The particles have a size of 1-10 mum that makes them particularly suitable for use in cell separation/sorting, cleaning biosubstances in suspension and diagnostic assays. The particles are prepared by dispersing a magnetic colloid containing a magnetic material such as a ferromagnetic or superparamagnetic substance in an aqueous solution of polyvinyl alcohol containing reactive hydroxyl groups, adding the resultant mixture to an organic phase containing a mixture of at least two emulsifiers, and adding a water-soluble cross-linking agent such as a dialdehyde that reacts with the hydroxyl groups of polyvinyl alcohol to form the polymer particles. The emulsifiers are miscible in the polyvinyl alcohol solution, and at least one emulsifier is semi-hydrophilic and at least one emulsifier is lipophilic. The cross-linking agent may be used in combination with a water-soluble diamine. The polymer particles contain reactive hydroxyl groups and can be provided with spacer molecules to which biomolecules such as antibodies, peptides, proteins, enzymes, streptavidin, avidin, oligonucleotides, oligosaccharides or DNA can be coupled. Vinyl monomers having one or more functional groups such as carboxyl, hydroxyl, amino, aldehyde or oxirane may be grafted to the polymer particles by radical polymerization under the catalytic effect of cerium (IV).