CATALYST TRANSFORMATION BY PROTEIN PHOSPHORYLATION AS A MEANS FOR ENZYME IMMUNOASSAY

A test composition for use in a specific binding enzyme immunoassay for detecting an analyte in a sample, includes: (a) a target protein capable of mediating a signal generating process, the mediating activity of the target protein being changed by covalently bonding a regulatory group thereto; (b)...

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Hauptverfasser: ROBERT H. ADOLFSEN, MOHAN S. SAINI, RANGA R. VUNNAM
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creator ROBERT H. ADOLFSEN
MOHAN S. SAINI
RANGA R. VUNNAM
description A test composition for use in a specific binding enzyme immunoassay for detecting an analyte in a sample, includes: (a) a target protein capable of mediating a signal generating process, the mediating activity of the target protein being changed by covalently bonding a regulatory group thereto; (b) a regulatory enzyme capable of catalyzing the covalent bonding of the regulatory group to the target protein; (c) a conjugate of a substrate molecule for the regulatory enzyme and an analyte, the substrate containing the regulatory group; (d) a specific binding partner for the analyte; and (e) reagent means for generating a detectable signal responsive to the condition of the target protein.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
title CATALYST TRANSFORMATION BY PROTEIN PHOSPHORYLATION AS A MEANS FOR ENZYME IMMUNOASSAY
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