CATALYST TRANSFORMATION BY PROTEIN PHOSPHORYLATION AS A MEANS FOR ENZYME IMMUNOASSAY

A test composition for use in a specific binding enzyme immunoassay for detecting an analyte in a sample, includes: (a) a target protein capable of mediating a signal generating process, the mediating activity of the target protein being changed by covalently bonding a regulatory group thereto; (b)...

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Bibliographische Detailangaben
Hauptverfasser:	ROBERT H. ADOLFSEN, MOHAN S. SAINI, RANGA R. VUNNAM
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A test composition for use in a specific binding enzyme immunoassay for detecting an analyte in a sample, includes: (a) a target protein capable of mediating a signal generating process, the mediating activity of the target protein being changed by covalently bonding a regulatory group thereto; (b) a regulatory enzyme capable of catalyzing the covalent bonding of the regulatory group to the target protein; (c) a conjugate of a substrate molecule for the regulatory enzyme and an analyte, the substrate containing the regulatory group; (d) a specific binding partner for the analyte; and (e) reagent means for generating a detectable signal responsive to the condition of the target protein.