FUNCTIONALLY INDEPENDENT CLONING VECTORS FOR USE IN STREPTOMYCETES

There is described a functionally independent recombinant DNA cloning vector comprising: a) a functional replicon-containing restriction fragment of plasmid pEL7 and b) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a sensitive host cell, and c) if d...

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Bibliographische Detailangaben
Hauptverfasser:	WALTER MITSUO NAKATSUKASA, JAMES ALBERT MABE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	There is described a functionally independent recombinant DNA cloning vector comprising: a) a functional replicon-containing restriction fragment of plasmid pEL7 and b) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a sensitive host cell, and c) if desired, a replicon that is functional in E. coli and a DNA segment that confers antibiotic resistance in a sensitive E. coli host cell, said host cells being susceptible to transformation, cell division, and culture.