NUCLEIC ACID HYBRIDIZATION PROBE

A nucleic acid hybridization method for detecting bacteria in a test sample, such as a mammalian body fluid (e.g., for detecting bacteriuria), employing a polynucleotide probe comprising a base sequence of at least a portion of one of the strands of a tuf or fus gene. Resulting hybridization between...

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Bibliographische Detailangaben
Hauptverfasser:	JOSEPHINE C. GROSCH, GARY A. WILSON
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A nucleic acid hybridization method for detecting bacteria in a test sample, such as a mammalian body fluid (e.g., for detecting bacteriuria), employing a polynucleotide probe comprising a base sequence of at least a portion of one of the strands of a tuf or fus gene. Resulting hybridization between the probe and single stranded genomic nucleic acids released from bacteria in the sample indicates bacterial presence and can be detected by employing labeled probes or otherwise detecting duplex formation. The method is particularly applicable to the screening of urine samples for the presence of bacteria belonging to the families Enterobacteriaceae, Psuedomonadaceae, and Streptococcaceae and particularly those of the genera Escherichia, Klebsiella, Enterobacter, Proteus, Psuedomonas, and Streptococcus. The method provides a unique, direct, and rapid means for detecting the presence of bacteria without the need for conventional microbiological culturing.