Probe, nucleic acid sequences, method particularly suitable for detecting and identifying yeasts, and genetic tools therefor

The detection and characterisation of yeasts, particularly those of medical and industrial interest, is disclosed. The aim is to provide powerful, reliable and simply and reproducibly usable DNA probes specific for numerous yeasts. For this purpose, among others, a yeast-specific and/or -infraspecif...

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Bibliographische Detailangaben
Hauptverfasser:	ARNAUD CARLOTTI, JEAN VILLARD
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The detection and characterisation of yeasts, particularly those of medical and industrial interest, is disclosed. The aim is to provide powerful, reliable and simply and reproducibly usable DNA probes specific for numerous yeasts. For this purpose, among others, a yeast-specific and/or -infraspecific detection probe is provided which consists of at least one part of at least one DNA and/or RNA fragment F from rDNA that does not code for rRNA synthesis and is located in the IGR (rDNA 25S and rDNA 18S). This iterative rDNA includes at least two repeated subsequences with a mutual homology of >/= 50 %. A detection method using said probe, as well as nucleic acid sequences capable of being included in the probe, are also disclosed. Said probe is useful for detecting/identifying yeast (e.g. C. krusei, G. candidum), as an epidemiological marker or strain tracer, and in anti-yeast therapy.