Novel bacillus thuringiensis genes coding toxins active against lepidopteran pests

The present invention is drawn to a novel toxin gene purified and isolated from Bacillus thuringiensis var kurstaki and given the designation CryIE(c). The novel toxin gene encodes a protein of approximately 130 kDa in size and is active against Lepidopteran insects. Also included in the invention a...

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Bibliographische Detailangaben
Hauptverfasser:	VANCE CARY KRAMER, THOMAS CURTIS CURRIER
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention is drawn to a novel toxin gene purified and isolated from Bacillus thuringiensis var kurstaki and given the designation CryIE(c). The novel toxin gene encodes a protein of approximately 130 kDa in size and is active against Lepidopteran insects. Also included in the invention are the proteins encoded by CryIE(c). Further disclosed are recombinant genes and proteins derived from CryIE(c). Also provided are biologically pure bacterial strains transformed with the CryIE(c) gene which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention are plants transformed with the toxin gene or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.