METHOD FOR PRODUCING RECOMBINANT HYALURONIDASE

METHOD FOR PRODUCING RECOMBINANT HYALURONIDASE

Disclosed is a method for producing hyaluronidase or a variant thereof. Specifically, the method is capable of changing the N-glycan levels under culture conditions including a controlled concentration of glucose in the culture medium and a decreased culture temperature for a specific culture time p...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Nam, Ki Seok, Kim, Kyuwan, Park, Kibum, Cho, Jeong Soo, Kim, Ji-Sun, Song, Hyung Nam, Yun, Sang Hoon, Byun, Minsoo, Park, Soon-Jae
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Disclosed is a method for producing hyaluronidase or a variant thereof. Specifically, the method is capable of changing the N-glycan levels under culture conditions including a controlled concentration of glucose in the culture medium and a decreased culture temperature for a specific culture time period, thereby increasing the specific activity by 10% or more and improving the quality and production yield. 300% soluble soluble variant (wild type)