CRISPR/Cpf1 systems and methods

"TTN" PAM Site Protospacer domain target binding site 5' -AAATTTAGGAAA ATTTTA HumanGenomicDNA HPRT1 i8595 Site TTTTAAATCCTTTCTCTTAACAAAAGAGGAAGGTCGT'GA ' uguagauggaaagagaauuguuuucuccuuc 3' u AsCpf1crRNA 0 ucaucuuuaau' This invention pertains to recombinant AsCPfl n...

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Bibliographische Detailangaben
Hauptverfasser:	COLLINGWOOD, Michael Allen, VAKULSKAS, Christopher Anthony, TURK, Rolf, BEHLKE, Mark Aaron
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	"TTN" PAM Site Protospacer domain target binding site 5' -AAATTTAGGAAA ATTTTA HumanGenomicDNA HPRT1 i8595 Site TTTTAAATCCTTTCTCTTAACAAAAGAGGAAGGTCGT'GA ' uguagauggaaagagaauuguuuucuccuuc 3' u AsCpf1crRNA 0 ucaucuuuaau' This invention pertains to recombinant AsCPfl nucleic acids and polypeptides for use in CRISPR/ Cpfl endonuclease sysems and mammalian cell lines encloding recombinant AsCpflor LbCpfl polypeptides. The invention includes recombinant ribonucleoprotein compleses and CRISPR/Cpfl endonuclease systems having a sjuitable AsCpfl or crRNA is selected from a length-truncated AsCpfl crRNA, a chemically modified AsCPfl crRNA, or an AsCpfl CrRNA comprising both length truncations and chemical modifications. Methods of performing gene editing using these system and reagents are also provided.