METHOD, KIT AND CARTRIDGE FOR DETECTING NUCLEIC ACID MOLECULE

A method for generating high accuracy sequencing results from low accuracy continuous single molecule sequencing includes the following steps: providing a sample including first target nucleic acid molecules; hybridizing first capture primers to the first target nucleic acid molecules 5 to form first capture primer-first target nucleic acid molecule complexes; performing a nascent strand synthesis reaction to form first template molecule-first target nucleic acid molecule complexes; dissociating first template molecules; hybridizing first sequencing primers to the dissociated first template molecules, and sequencing the first template molecules by single molecule sequencing to obtain first original sequences; comparing the first original sequences and 10 a first target sequence to calculate a first sequence similarity of each of the first original sequences; collecting first original sequences whose first sequence similarity reaches a predetermined threshold to obtain a first cluster; and analyzing the first cluster by bioinformatics to obtain a first consensus sequence. -31-