METHODS FOR PRODUCING NUCLEIC ACIDS

Described are methods for producing RNA molecules in an in vitro transcription reaction. Also described are methods for producing an mRNA molecule from a circular double- stranded DNA template using an in vitro transcription reaction system wherein the in vitro transcription reaction system lacks a...

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Bibliographische Detailangaben
Hauptverfasser:	VANDE VOORDE, Michael Casey, QUAGLIA MOTTA, Jennifer Julia, RAMESH, Athreya Subramanian, SWYERS, Michael Jay, BHATTACHARYA, Keshab, SMITH, Karenna Caroline, KIM, Sun Joong, AL-LOZI, Amer, WOOD, Nichole Lea, RUSSO, Joseph, REEVES, Erin Courtney, GHOSH, Swapnadip, SIMMS, Carrie Leanne, ALLISON, Daniel Ward, DONG, Guogang, SINANAN, Dillon Jarod, ALLEN, Martin John, JHUN, Hugo, PATEL, Urmi Ankit, SHOPERA, Tatenda, ZHENG, Xiaolu, ARMOUR, Kaley Elizabeth, MORAN, Justin Keith, WILLIAMSON, Jenna Kathryn, BREEN, Shelby Hutchins
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Described are methods for producing RNA molecules in an in vitro transcription reaction. Also described are methods for producing an mRNA molecule from a circular double- stranded DNA template using an in vitro transcription reaction system wherein the in vitro transcription reaction system lacks a polyamine. Also described are in vitro transcription reaction systems comprising enzymatic 5' capping and oligo(dT) purification.