METHODS, COMPOSITIONS AND KITS FOR INHIBITING FORMATION OF ADAPTER DIMERS

Methods of reducing adapter-dimers in a sequencing library are provided according to aspects of the present disclosure, including: hybridizing excess 3' adapters to blocker oligonucleotides forming a hybridized complex, such that the excess 3' adapters are unavailable to form adapter-dimer...

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Bibliographische Detailangaben
Hauptverfasser:	CASTRO, Eric, GUNNING, Kerry, LABAS, Allyson, ALLEN, Kevin D
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods of reducing adapter-dimers in a sequencing library are provided according to aspects of the present disclosure, including: hybridizing excess 3' adapters to blocker oligonucleotides forming a hybridized complex, such that the excess 3' adapters are unavailable to form adapter-dimers, the hybridized complex including: a first blocker oligonucleotide 3' terminus adjacent to an adenylated nucleotide at the 5' terminus of a first unligated 3' adapter and a second blocker oligonucleotide 3' terminus adjacent to an adenylated nucleotide at the 5' terminus of a second unligated 3' adapter, wherein the first blocker oligonucleotide has a 5' portion complementary to and hybridized to the first unligated 3' adapter, the second blocker oligonucleotide has a 5' portion complementary to and hybridized to the second unligated 3' adapter, and the first blocker oligonucleotide has a 3' portion complementary to, and hybridized to, a 3' portion of the second blocker oligonucleotide. The first and second blocker oligonucleotides may be the same oligonucleotide comprising a palindromic sequence which allows for self-hybridisation.