OFF-TARGET BLOCKING SEQUENCES TO IMPROVE TARGET DISCRIMINATION BY POLYMERASE CHAIN REACTION

Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR), comprising a labeled probe comprising a fluorophore and a quencher that is complementarity to and specifically binds to the target sequence and may non-specifical...

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Bibliographische Detailangaben
Hauptverfasser: KANE, Sarah, BARBARI, Stephanie, NASH, Rose, RAVET, Karl
Format: Patent
Sprache:eng
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