OFF-TARGET BLOCKING SEQUENCES TO IMPROVE TARGET DISCRIMINATION BY POLYMERASE CHAIN REACTION

Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR), comprising a labeled probe comprising a fluorophore and a quencher that is complementarity to and specifically binds to the target sequence and may non-specifical...

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Bibliographische Detailangaben
Hauptverfasser:	KANE, Sarah, BARBARI, Stephanie, NASH, Rose, RAVET, Karl
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR), comprising a labeled probe comprising a fluorophore and a quencher that is complementarity to and specifically binds to the target sequence and may non-specifically bind to the reference sequence and a promiscuity-blocking nucleotide juror oligonucleotide (PBNJ) that specifically binds to the reference sequence and may non-specifically bind to the target sequence, wherein the PBNJ comprises a reference binding region and an extension blocker that prevents elongation by a polymerase.