OFF-TARGET BLOCKING SEQUENCES TO IMPROVE TARGET DISCRIMINATION BY POLYMERASE CHAIN REACTION
Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR), comprising a labeled probe comprising a fluorophore and a quencher that is complementarity to and specifically binds to the target sequence and may non-specifical...
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| creator | KANE, Sarah BARBARI, Stephanie NASH, Rose RAVET, Karl |
| description | Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR), comprising a labeled probe comprising a fluorophore and a quencher that is complementarity to and specifically binds to the target sequence and may non-specifically bind to the reference sequence and a promiscuity-blocking nucleotide juror oligonucleotide (PBNJ) that specifically binds to the reference sequence and may non-specifically bind to the target sequence, wherein the PBNJ comprises a reference binding region and an extension blocker that prevents elongation by a polymerase. |
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BARBARI, Stephanie ; NASH, Rose ; RAVET, Karl</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_AU2022375767A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KANE, Sarah</creatorcontrib><creatorcontrib>BARBARI, Stephanie</creatorcontrib><creatorcontrib>NASH, Rose</creatorcontrib><creatorcontrib>RAVET, Karl</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KANE, Sarah</au><au>BARBARI, Stephanie</au><au>NASH, Rose</au><au>RAVET, Karl</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>OFF-TARGET BLOCKING SEQUENCES TO IMPROVE TARGET DISCRIMINATION BY POLYMERASE CHAIN REACTION</title><date>2024-05-02</date><risdate>2024</risdate><abstract>Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR), comprising a labeled probe comprising a fluorophore and a quencher that is complementarity to and specifically binds to the target sequence and may non-specifically bind to the reference sequence and a promiscuity-blocking nucleotide juror oligonucleotide (PBNJ) that specifically binds to the reference sequence and may non-specifically bind to the target sequence, wherein the PBNJ comprises a reference binding region and an extension blocker that prevents elongation by a polymerase.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | OFF-TARGET BLOCKING SEQUENCES TO IMPROVE TARGET DISCRIMINATION BY POLYMERASE CHAIN REACTION |
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