ENGINEERED TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE VARIANTS

The present invention provides engineered terminal deoxynucleotidyl transferase (TdT) polypeptides useful in template-independent polynucleotide synthesis capable of adding a nucleoside triphosphate-3' - O-removable blocking group (NTP-3'-O-RBG) to the 3'-OH end of a growing oligonucl...

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Bibliographische Detailangaben
Hauptverfasser:	FORGET, Stephanie, MAYO, Melissa, KRAWCZYK, Mikayla, MAHMOODI, Niusha, CHING, Charlene, KNIGHT, Anders, MILLER, Mathew, VROOM, Jonathan
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides engineered terminal deoxynucleotidyl transferase (TdT) polypeptides useful in template-independent polynucleotide synthesis capable of adding a nucleoside triphosphate-3' - O-removable blocking group (NTP-3'-O-RBG) to the 3'-OH end of a growing oligonucleotide or polynucleotide chain in a template-independent manner, as well as compositions, methods of utilizing these engineered polypeptides, and polynucleotides encoding the engineered terminal deoxynucleotidyl transferase.