CELLULAR ENGINEERING TO IMPROVE CANNABINOID PRODUCTION IN MICROBIAL CELLS

Provided herein are recombinant enzymes, cells, and methods to optimize the production of cannabinoids in micro-organisms. In particular, the cells produce an increased ratio of geranyl diphosphate (GPP) to farnesyl diphosphate (FPP) as compared to a control cell, and the cell expresses a mutant far...

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Bibliographische Detailangaben
Hauptverfasser:	URANO, Jun, NGUYEN, Diep, COLLINS, Carolyn, KAMBOURAKIS, Spiros, CAIAZZA, Nicky
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided herein are recombinant enzymes, cells, and methods to optimize the production of cannabinoids in micro-organisms. In particular, the cells produce an increased ratio of geranyl diphosphate (GPP) to farnesyl diphosphate (FPP) as compared to a control cell, and the cell expresses a mutant farnesyl pyrophosphate synthase protein (FPPS). This mutant FPPS comprises one or more insertion, deletion, or substitution at an amino acid position equivalent to one or more amino acid positions 88-90 of wild-type ERG20. in addition, the cell may express or overexpress one or more enzymes in a synthesis pathway of one or more monoterpenes, prenylated compounds, or cannabinoids.