Automated production and collection

Embodiments described herein provide for the production, isolation, and/or collection of cellular product(s) released or secreted from cells. Cells may be expanded in the intracapillary (or extracapillary) space of a bioreactor of a cell expansion system with media. Cells may release cellular products into the fluid space of the bioreactor. Examples of such released cellular products include extracellular particles, such as extracellular vesicles (EVs). To collect the extracellular particles released from the cells being expanded, as opposed to any extracellular particles from other sources, a washout procedure may be used to eliminate any serum proteins prior to collecting the released extracellular particles from the expanding cells. The released cellular products may be collected or concentrated through the control of outlet parameters, while nutrients may reach the cells through the diffusion of media through a semi-permeable membrane, for example. The released cellular products may then be harvested.