DEVICES AND METHODS FOR NUCLEIC ACID IDENTIFICATION IN SAMPLES

A device, method, and non-transitory computer readable medium storing instructions for nucleic acid identification of material in a sample includes a microfluidics system, including a movable cartridge, and a heat source. The movable cartridge assembly includes at least one target-specific set of re...

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Bibliographische Detailangaben
Hauptverfasser:	SATTERLY, Neal G, PFEUFFER, Kevin P
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A device, method, and non-transitory computer readable medium storing instructions for nucleic acid identification of material in a sample includes a microfluidics system, including a movable cartridge, and a heat source. The movable cartridge assembly includes at least one target-specific set of reagent components and a set of RCA-LAMP reaction components deposited on the surface. Moreover, the at least one target-specific set of reagent components includes at least one target specific padlock probe reagent component. The set of RCA-LAMP reaction components includes: at least one polymerase buffer component, at least one polymerase enzyme with strand displacement activity, a betaine additive, a TETRONIC additive, a sequence-specific probe, dNTPs, and a primer mix. The primer mix includes both a forward inside primer and a backward inside primer specific to a backbone of the at least one target specific padlock probe reagent component.