SYSTEM AND METHOD FOR PERFORMING A MICROBIOME-ANALYSIS

The present invention relates to a method for performing a microbiome-analysis, comprising the steps: a) providing a primary sample derived from a microbiomic sample, the primary sample comprising a first target species at a first concentration and a second target species at a second concentration and different from the first target species, wherein the first target species is indicative of a first element of a microbiome of the microbiomic sample and the second target species is indicative of a second element of the microbiome, and wherein the second concentration is higher than the first concentration; b) providing two or more secondary samples derived from the primary sample, the two or more secondary samples including at least a highest dilution secondary sample, a lowest dilution secondary sample and optionally one or more intermediate dilution secondary samples, wherein the at least two secondary samples have different dilutions, and wherein the lowest dilution secondary sample may be diluted or undiluted primary sample; c) providing a first fluorescent label that is configured to directly or indirectly bind specifically to the first target species to the lowest dilution secondary sample under conditions allowing for labeling the first target species with the first fluorescent label, and providing a second fluorescent label that is configured to directly or indirectly bind specifically to the second target species to the highest dilution secondary sample, but not to the lowest dilution secondary sample, under conditions allowing for labeling the second target species with the second fluorescent label; d) analyzing the first and second target species according to the steps: dl) imaging the secondary samples with fluorescence microscopy and counting the fluorescent first and second labels in the corresponding secondary samples; d2) calculating the ratio of the first target species to the second target species from the results of step dl) and the dilutions of the corresponding secondary samples.