Thermostable terminal deoxynucleotidyl transferase

Thermostable terminal deoxynucleotidyl transferase

Disclosed herein include recombinant terminal deoxynucleotidyl transferases (TdTs). In some embodiments, the recombinant TdT comprises an amino acid sequence that is at least 80% identical to a bovine TdT, wherein the recombinant TdT comprises one or more amino acid substitution mutations at one or...

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Bibliographische Detailangaben
Hauptverfasser: CHUA, Jasmine Puay Susan, PEISAJOVICH, Sergio, YEW, Wen Shan, GO, Maybelle Darlene Kho, NIRANTAR, Saurabh Rajendra
Format: Patent
Sprache:eng
Schlagworte:
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
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MICROBIOLOGY
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Zusammenfassung:Disclosed herein include recombinant terminal deoxynucleotidyl transferases (TdTs). In some embodiments, the recombinant TdT comprises an amino acid sequence that is at least 80% identical to a bovine TdT, wherein the recombinant TdT comprises one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His421 in the bovine TdT.