Improved processes for in vitro transcription of messenger RNA

The present invention provides methods for preparing optimized DNA sequences as templates for in vitro transcription of mRNA. These DNA sequences are optimized to avoid premature termination of transcription by RNA polymerase. The invention also provides methods for preparing optimized DNA sequences...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: TRAN, Khang Anh, DUBINS, Jeffrey S, COOPER, Dustin, GU, Xiaobo, DEROSA, Frank, DIAS, Anusha
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention provides methods for preparing optimized DNA sequences as templates for in vitro transcription of mRNA. These DNA sequences are optimized to avoid premature termination of transcription by RNA polymerase. The invention also provides methods for preparing optimized DNA sequences that include one or more termination signal at their 3' end to reduce or prevent non-templated "runoff" transcription.