Capturing genetic targets using a hybridization approach

Provided herein are methods of determining a location of an analyte using hybridization as a method for enhancing detection of the analyte. In particular, capture probes comprising a spatial barcode and a capture domain are used to capture analytes in a biological sample contacted with a substrate....

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Bibliographische Detailangaben
Hauptverfasser:	PFEIFFER, Katherine, DELANEY, Nigel, MESCHI, Francesca, BENT, Zachary, CHEW, Jennifer, HILL, Andrew John, ALVARADO MARTINEZ, Luigi Jhon, KOHLWAY, Andrew Scott, ARTHUR, Joseph Glenn
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided herein are methods of determining a location of an analyte using hybridization as a method for enhancing detection of the analyte. In particular, capture probes comprising a spatial barcode and a capture domain are used to capture analytes in a biological sample contacted with a substrate. The analyte may be a nucleic acid or a protein. Bait oligonucleotides are used to enrich a nucleic acid of interest before sequencing.