Purification of saccharides

The present invention relates to methods for purifying bacterial polysaccharides, in particular for removing impurities from cellular lysates of bacteria producing polysaccharides, comprising: a) acid hydrolysis; b) a first ultrafiltration/diafiltration-(UFDF-1); b) carbon filtration; c) chromatogra...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MERCHANT, Nishith, CHU, Ling, MORAN, Justin Keith, CHEN, Wei
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention relates to methods for purifying bacterial polysaccharides, in particular for removing impurities from cellular lysates of bacteria producing polysaccharides, comprising: a) acid hydrolysis; b) a first ultrafiltration/diafiltration-(UFDF-1); b) carbon filtration; c) chromatography; and d) a second ultrafiltration/diafiltration-(UFDF-2).