Devices and methods for transfection and for generation of clonal populations of cells

Disclosed herein are cartridges for transfecting cells and/or generating clonal populations of cells comprising: a) a first compartment configured for performing cell transfection, wherein the first compartment comprises a first inlet configured for introduction of a cell sample; b) a second compart...

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Hauptverfasser: BROWN, Alexander, WHITNEY, Brandon, DABROWSKI, Paul, KHOSROSHAHI, Maryam, RAZINKOV, Ivan, KELSO, Reed
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creator BROWN, Alexander
WHITNEY, Brandon
DABROWSKI, Paul
KHOSROSHAHI, Maryam
RAZINKOV, Ivan
KELSO, Reed
description Disclosed herein are cartridges for transfecting cells and/or generating clonal populations of cells comprising: a) a first compartment configured for performing cell transfection, wherein the first compartment comprises a first inlet configured for introduction of a cell sample; b) a second compartment configured for performing cell selection, wherein an inlet of the second compartment is operably coupled to an outlet of the first compartment, and wherein the second compartment further comprises at least one optically-transparent wall and an outlet that is operably coupled to an intermediate cell removal port; and c) a third compartment configured for performing cell expansion, wherein an inlet of the third compartment is operably coupled to the outlet of the second compartment.
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subjects APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRANSPORTING
VINEGAR
WINE
title Devices and methods for transfection and for generation of clonal populations of cells
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