Devices and methods for transfection and for generation of clonal populations of cells

Disclosed herein are cartridges for transfecting cells and/or generating clonal populations of cells comprising: a) a first compartment configured for performing cell transfection, wherein the first compartment comprises a first inlet configured for introduction of a cell sample; b) a second compart...

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Bibliographische Detailangaben
Hauptverfasser:	BROWN, Alexander, WHITNEY, Brandon, DABROWSKI, Paul, KHOSROSHAHI, Maryam, RAZINKOV, Ivan, KELSO, Reed
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed herein are cartridges for transfecting cells and/or generating clonal populations of cells comprising: a) a first compartment configured for performing cell transfection, wherein the first compartment comprises a first inlet configured for introduction of a cell sample; b) a second compartment configured for performing cell selection, wherein an inlet of the second compartment is operably coupled to an outlet of the first compartment, and wherein the second compartment further comprises at least one optically-transparent wall and an outlet that is operably coupled to an intermediate cell removal port; and c) a third compartment configured for performing cell expansion, wherein an inlet of the third compartment is operably coupled to the outlet of the second compartment.