Methods, reagents and kits for flow cytometric immunophenotyping

Abstract The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least three identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions. WO 2010/140885 PCT/NL2010/050332 Figure 1 EuroFlow panels of antibody combinations Category 1 Category 2 - Category 3 Mature lymphoid panel Immature lymphoid panel Myeloid panel Single otube screening combinations Single tube screening combination Single tube screening combination -LST -ALOT ALOT -PCST Multi-tube classification combinations Multi-tube classification combinations Multi-tube classification combination B-CLPD BCP-ALL AML/MDS/MPD* - T-CLPD - T-ALL also suited or: - NK-CLPD mastucytosis -PCD PNH bIus Figure 2 Acute leukeocti Suspect smallcell samples Monoclonal Involvement (e.g.SF, FNA. vitreous) component Sustainednon-igM ntoi Monoconal on-M - 1. eactive/pelyctonail other ->B-CL PD (clonat) B ALL T-ALL AML/MDS B C D B-bG LD-LPD NKCLPD PCD CLL ) CLL aberrant) aberr t ur terizatln FBCP-AL s tPes non-CL MCL aberrant reactive acIll ofTALL uuAM MOB ~reactive? acy -eother clonal B -CML -CML-BC) -other MPOD