A METHOD OF VACUUM INFILTRATION ASSISTING AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF SUGARCANE

The present disclosure relates to the technical field of plant genetic engineering, and discloses a method of vacuum infiltration assisting Agrobacterium-mediated genetic transformation of sugarcane, which comprises the following steps: (1) induction and proliferation of embryogenic callus from sugarcane heart leaf; (2) activation and cultivation of Agrobacterium containing the target gene; (3) vacuum infiltration assisting Agrobacterium infection of sugarcane embryogenic callus; (4) screening and emergence of resistant materials; (5) identification of the resistant materials. The method of vacuum infiltration assisting Agrobacterium-mediated genetic transformation of sugarcane used in the present disclosure can prompt Agrobacterium to transfer into deep cells through the gaps between callus cells under vacuum, and cause small wounds on the surface of the cells, resulting in plants secrete some phenolic substances to activate the shearing and transfer of T-DNA in the Ti plasmid. The present disclosure improves the Agrobacterium-mediated genetic transformation system of sugarcane. Compared with the common Agrobacterium-mediated method, the method in the present disclosure significantly improves the genetic transformation rate of sugarcane. Control Transgenic plant obtained with the method in the present disclosure Control Transgenic plant obtained with the method in the present disclosure